Anisotropic Bone Surface Topography Mimicked Chitosan/Graphene Oxide Membranes

Synergy between biomaterial surfaces and cells is known to be important due the direct inevitable interactions that mediate cell behavior. Thus, design of biomimetic with proper topography chemistry crucial for optimization cellular responses. Herein, we report surface mimicking ability chitosan (CH) biopolymer its promising application as a platform osteoblast culture. CH frequently used in bone tissue engineering applications. For this reason, anisotropic was chosen demonstrate skill. Initially, replicated by using soft lithography polydimethylsiloxane (PDMS) molds. Subsequently, solvent casting performed on molds, then polymer membranes are obtained. To prepare nanocomposite, graphene oxide (GO) blended into enhance biocompatibility. It observed CH/GO nanocomposite both eligible mimic surface. Considering tissue, hydroxyapatite (HA) modification also conducted ultraviolet/ozone method. Following that, human osteoblasts evaluate responses mimicked surfaces. The results indicate has positive impact viability morphology.